바이칼레인(baicalein)이 peptidoglycan으로 자극된 RAW 264.7 mouse macrophages의 hydrogen peroxide 생성에 미치는 영향Effects of Baicalein on hydrogen peroxide productions in RAW 264.7 mouse macrophages stimulated by peptidoglycan
- Other Titles
- Effects of Baicalein on hydrogen peroxide productions in RAW 264.7 mouse macrophages stimulated by peptidoglycan
- Authors
- 박완수
- Issue Date
- Jan-2023
- Publisher
- 대한본초학회
- Keywords
- RAW 264.7; Baicalein; Macrophage; Peptidoglycan; Hydrogen peroxide
- Citation
- 대한본초학회지, v.38, no.1, pp.1 - 9
- Journal Title
- 대한본초학회지
- Volume
- 38
- Number
- 1
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/87264
- ISSN
- 1229-1765
- Abstract
- Objectives : The aim of this study was to investigate the effect of baicalein (BA) on the production of hydrogen peroxide in peptidoglycan-stimulated RAW 264.7 mouse macrophages.
Methods : Peptidoglycan-stimulated RAW 264.7 were incubated with baicalein at concentrations of 50 and 100 µM. Incubation time is 30 min, 2 h, 12 h, and 18 h. After incubation, The production of hydrogen peroxide in RAW 264.7 was measured with dihydrorhodamine 123 assay. Berberine and gallic acid were used as the comparative materials.
Results : BA at the concentration of 50 and 100 µM did not show cytotoxicity on RAW 264.7 for 24 h incubation. For 30 min, 2 h, 12 h, and 18 h incubation, BA at the concentration of 50 and 100 µM significantly inhibited the production of hydrogen peroxide in RAW 264.7 stimulated by peptidoglycan (p < 0.05). In details, production of hydrogen peroxide in peptidoglycan-stimulated RAW 264.7 treated for 30 min with BA at concentrations of 50 and 100 µM was 93.91% and 93.52% of the control group treated with peptidoglycan only, respectively; the production of hydrogen peroxide for 2 h was 93.8% and 92.71%, respectively; production of hydrogen peroxide for 12 h was 94.86% and 95.93%, respectively; production of hydrogen peroxide for 18 h was 95.37% and 96.48%, respectively.
Conclusions : BA might have anti-oxidative activity related to its inhibition of hydrogen peroxide production in peptidoglycan-stimulated RAW 264.7 macrophages.
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