Identification and mechanism of action of renoprotective constituents from peat moss Sphagnum palustre in cisplatin-induced nephrotoxicity
- Authors
- Kang, Hee Rae; Lee, Dahae; Eom, Hee Jeong; Lee, Seoung Rak; Lee, Kang Ro; Kang, Ki Sung; Kim, Ki Hyun
- Issue Date
- Jan-2016
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Sphagnum palustre; Sphagnaceae; Flavonoid; Structural elucidation; Nephrotoxicity; MAPKs
- Citation
- JOURNAL OF FUNCTIONAL FOODS, v.20, pp.358 - 368
- Journal Title
- JOURNAL OF FUNCTIONAL FOODS
- Volume
- 20
- Start Page
- 358
- End Page
- 368
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8747
- DOI
- 10.1016/j.jff.2015.11.010
- ISSN
- 1756-4646
- Abstract
- In search for bioactive constituents from natural resources, the ethanol (EtOH) extract of Sphagnum palustre showed significant renoprotective effects against cisplatin-induced damage in kidney cells. Phytochemical investigation of the EtOH extract led to the identification of a new flavonoid, 6-methyl-(2R,35)-alpinone, along with six known flavonoids, four steroids, six triperpenoids, and three fatty acids. Among them, ergosterol peroxide, (3 beta,22E,24S)-3-hydroxy-ergosta-5,22-dien-7-one, and betulinic acid ameliorated cisplatin-induced nephrotoxicity to 80% of the control value at 125, 125 and 50 mu M, respectively. Moreover, the elevated percentage of apoptotic cells by cisplatin was significantly reduced after co-treatment with the EtOH extract of S. palustre and ergosterol peroxide, (3 beta,22E,24S)-3-hydroxy-ergosta-5,22-dien-7-one, and betulinic acid. Upregulated phosphorylation of INK and p38 by cisplatin treatment was markedly decreased after co-treatment with ergosterol peroxide, (3 beta,22E,24S)-3-hydroxy-ergosta-5,22-dien-7-one, and betulinic acid. These results show that blocking the MAPKs signalling cascade plays a critical role in mediating the renoprotective effect of S. palustre. (C) 2015 Elsevier Ltd. All rights reserved.
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