Polyphenolic Carbon Quantum Dots with Intrinsic Reactive Oxygen Species Amplification for Two-Photon Bioimaging and In Vivo Tumor Therapy
- Authors
- Dutta, Sayan Deb; Moniruzzaman, Md; Hexiu, Jin; Sarkar, Sourav; Ganguly, Keya; Patel, Dinesh K.; Mondal, Jagannath; Lee, Yong-Kyu; Acharya, Rumi; Kim, Jongsung; Lim, Ki-Taek
- Issue Date
- Oct-2023
- Publisher
- AMER CHEMICAL SOC
- Keywords
- polyphenolic CQDs; mitochondrial dysfunctions; two-photon imaging; oxidative stress; nanotheranostics
- Citation
- ACS APPLIED MATERIALS & INTERFACES, v.15, no.45, pp 52083 - 52099
- Pages
- 17
- Journal Title
- ACS APPLIED MATERIALS & INTERFACES
- Volume
- 15
- Number
- 45
- Start Page
- 52083
- End Page
- 52099
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/89518
- DOI
- 10.1021/acsami.3c07547
- ISSN
- 1944-8244
1944-8252
- Abstract
- Recent studies indicate that mitochondrial dysfunctions and DNA damage have a critical influence on cell survival, which is considered one of the therapeutic targets for cancer therapy. In this study, we demonstrated a comparative study of the effect of polyphenolic carbon quantum dots (CQDs) on in vitro and in vivo antitumor efficacy. Dual emissive (green and yellow) shape specific polyphenolic CQDs (G-CQDs and Y-CQDs) were synthesized from easily available nontoxic precursors (phloroglucinol), and the antitumor property of the as-synthesized probe was investigated as compared to round-shaped blue emissive CQDs (B-CQDs) derived from well-reported precursor citric acid and urea. The B-CQDs had a nuclei-targeting property, and G-CQDs and Y-CQDs had mitochondria-targeting properties. We have found that the polyphenol containing CQDs (at a dose of 100 mu g mL(-1)) specifically attack mitochondria by excess accumulation, altering the metabolism, inhibiting branching pattern, imbalanced Bax/Bcl-2 homeostasis, and ultimately generating oxidative stress levels, leading to oxidative stress-induced cell death in cancer cells in vitro. We show that G-CQDs are the main cause of oxidative stress in cancer cells because of their ability to produce sufficient (OH-)-O-center dot and O-1(2) radicals, evidenced by electron paramagnetic resonance spectroscopy and a terephthalic acid test. Moreover, the near-infrared absorption properties of the CQDs were exhibited in two-photon (TP) emission, which was utilized for TP cellular imaging of cancer cells without photobleaching. The in vivo antitumor test further discloses that intratumoral injection of G-CQDs can significantly augment the treatment efficacy of subcutaneous tumors without any adverse effects on BalB/c nude mice. We believe that shape-specific polyphenolic CQD-based nanotheranostic agents have a potential role in tumor therapy, thus proving an insight on treatment of malignant cancers.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/89518)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.