Organoid-Based Human Stomach Micro-Physiological System to Recapitulate the Dynamic Mucosal Defense Mechanismopen access
- Authors
- Jeong, Hye-Jin; Park, Ji-Hyeon; Kang, Joo H. H.; Sabate del Rio, Jonathan; Kong, Seong-Ho; Park, Tae-Eun
- Issue Date
- Sep-2023
- Publisher
- WILEY
- Keywords
- gastric mucosal defense mechanism; Helicobacter pylori; human gastric organoids; micro-physiological system; telocytes; Trefoil factor 1
- Citation
- ADVANCED SCIENCE, v.10, no.27
- Journal Title
- ADVANCED SCIENCE
- Volume
- 10
- Number
- 27
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/89663
- DOI
- 10.1002/advs.202300164
- ISSN
- 2198-3844
2198-3844
- Abstract
- Several stomach diseases are attributed to the dysregulation of physiological function of gastric mucosal barrier by pathogens. Gastric organoids are a promising tool to develop treatment strategies for gastric infections. However, their functional features of in vivo gastric mucosal barrier and host-microbe interactions are limited due to the lack of physiological stimuli. Herein, a human stomach micro-physiological system (hsMPS) with physiologically relevant gastric mucosal defense system is described based on the combination of organoid and MPS technology. A fluid flow enhanced epithelial-mesenchymal interaction in the hsMPS enables functional maturation of gastric epithelial cells, which allows for the recreation of mesh-like mucus layer containing high level of mucus protective peptides and well-developed epithelial junctional complexes. Furthermore, gastroprotection mechanisms against Helicobacter pylori (H. pylori) are successfully demonstrated in this system. Therefore, hsMPS represents a new in vitro tool for research where gastric mucosal defense mechanism is pivotal for developing therapeutic strategies.
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