A liquid crystal-based biosensor for sensitive detection of tumor necrosis factor-alpha
- Authors
- Ryu, Je-Jin; Jang, Chang-Hyun
- Issue Date
- Jan-2024
- Publisher
- SPRINGER WIEN
- Keywords
- Liquid crystal; Biosensor; Tumor necrosis factor-alpha; Antibody; Polarized optical microscope
- Citation
- MICROCHIMICA ACTA, v.191, no.1
- Journal Title
- MICROCHIMICA ACTA
- Volume
- 191
- Number
- 1
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/90215
- DOI
- 10.1007/s00604-023-06125-y
- ISSN
- 0026-3672
1436-5073
- Abstract
- Tumor necrosis factor-alpha (TNF-alpha) is a cytokine secreted by the macrophages and Th1 cells of the immune system in response to inflammation. Given its significance as a biomarker with elevated levels in physiological fluids in various conditions, there is an increasing demand for a simple and accurate TNF-alpha detection strategy. In this article, we present a liquid crystal (LC)-based biosensor developed for sensitive TNF-alpha detection. The biosensor operates as follows: TNF-alpha and detection antibodies (DAbs) form complexes during preincubation. These complexes then bind with the surface-immobilized capture antibodies (CAbs), facilitating the antigen-antibody reaction between the CAbs and the TNF-alpha/DAb complexes. This target recognition interaction alters the surface topography, disrupting the vertical orientation of LCs produced by dimethyloctadecyl[3-(trimethoxysilyl)-propyl]ammonium chloride. The orientational change in the LCs can be easily visualized with a polarized optical microscope, resulting in brighter images as TNF-alpha levels rise. Our results demonstrated a linear range of 5.00-500 pg/mL, with a limit of detection and limit of quantification being 1.08 and 3.56 pg/mL, respectively. Recovery experiments on diluted saliva samples produced reasonable results, with TNF-alpha recoveries ranging from 97.1% +/- 2.58% to 107% +/- 5.95%.
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