Novel Enzymatic Crosslinking-based Hydrogel Nanofilm Caging System on Pancreatic β-cell Spheroid for Long-term Blood Glucose Regulationopen access
- Authors
- Kim, Minji; Kim, Hyunbum; Lee, Young-sun; Lee, Sangjun; Kim, Seong-Eun; Lee, Uk-Jae; Jung, Sungwon; Park, Chung-Gyu; Hong, Jinkee; Doh, Junsang; Lee, Dong Yun; Kim, Byung-Gee; Hwang, Nathaniel S.
- Issue Date
- Jun-2021
- Publisher
- AMER ASSOC ADVANCEMENT SCIENCE
- Citation
- SCIENCE ADVANCES, v.7, no.26, pp.1 - 10
- Indexed
- SCIE
SCOPUS
- Journal Title
- SCIENCE ADVANCES
- Volume
- 7
- Number
- 26
- Start Page
- 1
- End Page
- 10
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/1025
- DOI
- 10.1126/sciadv.abf7832
- ISSN
- 2375-2548
- Abstract
- Pancreatic β cell therapy for type 1 diabetes is limited by low cell survival rate owing to physical stress and aggressive host immune response. In this study, we demonstrate a multilayer hydrogel nanofilm caging strategy capable of protecting cells from high shear stress and reducing immune response by interfering cell-cell interaction. Hydrogel nanofilm is fabricated by monophenol-modified glycol chitosan and hyaluronic acid that cross-link each other to form a nanothin hydrogel film on the cell surface via tyrosinase-mediated reactions. Furthermore, hydrogel nanofilm formation was conducted on mouse β cell spheroids for the islet transplantation application. The cytoprotective effect against physical stress and the immune protective effect were evaluated. Last, caged mouse β cell spheroids were transplanted into the type 1 diabetes mouse model and successfully regulated its blood glucose level. Overall, our enzymatic cross-linking–based hydrogel nanofilm caging method will provide a new platform for clinical applications of cell-based therapies.
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