Role of MicroRNA-34a in Anti-Apoptotic Effects of Granulocyte-Colony Stimulating Factor in Diabetic Cardiomyopathyopen access
- Authors
- Park, In-Hwa; Song, Yi-Sun; Joo, Hyun-Woo; Shen, Guang-Yin; Seong, Jin-Hee; Shin, Na-Kyoung; Cho, Young Jong; Lee, Yonggu; Shin, Jeong Hun; Lim, Young-Hyo; Kim, Hyuck; Kim, Kyung-Soo
- Issue Date
- Feb-2020
- Publisher
- KOREAN DIABETES ASSOC
- Keywords
- Diabetic cardiomyopathies; Granulocyte colony-stimulating factor; MicroRNAs
- Citation
- DIABETES & METABOLISM JOURNAL, v.44, no.1, pp.173 - 185
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- DIABETES & METABOLISM JOURNAL
- Volume
- 44
- Number
- 1
- Start Page
- 173
- End Page
- 185
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/10787
- DOI
- 10.4093/dmj.2018.0211
- ISSN
- 2233-6079
- Abstract
- Background: Recent studies have shown that microRNAs (miRNAs) are involved in the process of cardiomyocyte apoptosis. We have previously reported that granulocyte-colony stimulating factor (G-CSF) ameliorated diastolic dysfunction and attenuated cardiomyocyte apoptosis in a rat model of diabetic cardiomyopathy. In this study, we hypothesized a regulatory role of cardiac miRNAs in the mechanism of the anti-apoptotic effect of G-CSF in a diabetic cardiomyopathy rat model.
Methods: Rats were given a high-fat diet and low-dose streptozotocin injection and then randomly allocated to receive treatment with either G-CSF or saline. H9c2 rat cardiomyocytes were cultured under a high glucose (HG) condition to induce diabetic cardiomyopathy in vitro. We examined the extent of apoptosis, miRNA expression, and miRNA target genes in the myocardium and H9c2 cells.
Results: G-CSF treatment significantly decreased apoptosis and reduced miR-34a expression in diabetic myocardium and H9c2 cells under the HG condition. G-CSF treatment also significantly increased B-cell lymphoma 2 (Bcl-2) protein expression as a target for miR-34a. In addition, transfection with an miR-34a mimic significantly increased apoptosis and decreased Bcl-2 luciferase activity in H9c2 cells.
Conclusion: Our results indicate that G-CSF might have an anti-apoptotic effect through down-regulation of miR-34a in a diabetic cardiomyopathy rat model.
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