Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cellsopen access
- Authors
- Kim, Gae-Baik; Lee, Jeong Min; Nguyen, Duc Long; Lee, Joonseok; Kim, Young-Pil
- Issue Date
- Feb-2022
- Publisher
- MDPI
- Keywords
- activatable peptides; fluorescent imaging; proteases; MMP2; cancer cells
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.23, no.3, pp.1 - 12
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 23
- Number
- 3
- Start Page
- 1
- End Page
- 12
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/139528
- DOI
- 10.3390/ijms23031605
- ISSN
- 1661-6596
- Abstract
- Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide consists of anionic (EEEE), cleavable, and cationic sequences (RRRR) that enable intracellular delivery by matrix metalloproteinase-2 (MMP2), which is secreted by living cancer cells. Compared to HT-29 cells (MMP2-negative), HT-1080 cells (MMP2-positive) showed a strong fluorescence response to the short fluorescent peptide via cell-secreted protease activation. Our approach is expected to find applications for the rapid visualization of protease activity in living cells.
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Collections - 서울 자연과학대학 > 서울 생명과학과 > 1. Journal Articles
- 서울 자연과학대학 > 서울 화학과 > 1. Journal Articles
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