An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses
- Authors
- Gwak, Yunho; Jung, Woongsic; Lee, Yew; Kim, Ji Sook; Kim, Chul Geun; Ju, Ji-Hyun; Song, Chihong; Hyun, Jae-Kyung; Jin, EonSeon
- Issue Date
- Nov-2014
- Publisher
- FEDERATION AMER SOC EXP BIOL
- Keywords
- Chaetoceros neogracile; immunogold labeling; ice-binding site
- Citation
- FASEB JOURNAL, v.28, no.11, pp.4924 - 4935
- Indexed
- SCIE
SCOPUS
- Journal Title
- FASEB JOURNAL
- Volume
- 28
- Number
- 11
- Start Page
- 4924
- End Page
- 4935
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/143965
- DOI
- 10.1096/fj.14-256388
- ISSN
- 0892-6638
- Abstract
- The structure and function of the Antarctic marine diatom Chaetoceros neogracile antifreeze protein (Cn-AFP), as well as its expression levels and characteristics of the ice-binding site, were analyzed in the present study. In silico analysis revealed that the Cn-AFP promoter contains both light- and temperature-responsive elements. Northern and Western blot analyses demonstrated that both Cn-AFP transcript and protein expression were strongly and rapidly stimulated by freezing, as well as temperature and high light stress. Immunogold labeling revealed that Cn-AFP is preferentially localized to the intracellular space near the chloroplast membrane. Recombinant Cn-AFP had clear antifreeze activity. Protein-folding simulation was used to predict the putative ice-binding sites in Cn-AFP, and site-directed mutagenesis of the Cn-AFP b-face confirmed their identification.Gwak, Y., Jung, W., Lee, Y., Kim, J. S., Kim, C. G., Ju, J.-H., Song, C., Hyun, J.-K., Jin, E. An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses.
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