Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Ratsopen access
- Authors
- Joo, Hyun-Woo; Song, Yi-Sun; Park, In-Hwa; Shen, Guang-Yin; Seong, Jin-Hee; Shin, Na-Kyoung; Lee, A-Hyeon; Kim, Hyuck; Kim, Kyung-Soo
- Issue Date
- Jul-2020
- Publisher
- HINDAWI LTD
- Citation
- CANADIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, v.2020, pp.1 - 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- CANADIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
- Volume
- 2020
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/145456
- DOI
- 10.1155/2020/2156829
- ISSN
- 2291-2789
- Abstract
- Background. We previously reported that the granulocyte colony stimulating factor (G-CSF) ameliorated hepatic steatosis with the enhancement of beta-oxidation-related gene expression. However, the mechanisms underlying this process remain unclear. This study aimed to determine whether the improvement of hepatic steatosis by G-CSF was associated with autophagy in a rat model of diabetes.Methods. Eight rats were fed a standard diet, and 16 rats were fed high-fat diet (HFD) for 5 weeks. All HFD-fed rats were then injected with streptozotocin (STZ). One week later, HFD rats injected with STZ were randomly treated with either G-CSF (200 mu g/kg/day; diabetes mellitus (DM)/G-CSF) or saline (DM/saline) for 5 consecutive days. Four weeks later, serum biochemical and histology analyses were conducted. The expression of autophagy-associated proteins was determined by Western blotting. The mRNA expression of beta-oxidation-related genes was determined by quantitative real-time polymerase chain reaction. HepG2 cells were cultured under high glucose (HG) conditions with G-CSF treatment, followed by Oil Red O staining for quantification of lipids.Results. Histological analysis showed lower lipid accumulation in the DM/G-CSF group than in the DM/saline-treated rats. Protein levels of LC3 and beclin-1 were higher, and those of p62 were lower in the DM/G-CSF rats than in the DM/saline-treated rats. The mRNA expression of beta-oxidation-related genes was higher in DM/G-CSF rats than in the DM/saline-treated rats. Quantification of lipid levels in HepG2 cells cultured with HG and G-CSF treatment revealed no significant differences.Conclusions. Our data suggested that G-CSF potentially improves hepatic steatosis and autophagy in the liver of diabetic rats.
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