Optimization of tenocyte lineage-related factors from tonsil-derived mesenchymal stem cells using response surface methodologyopen access
- Authors
- Kwon, Soon-Sun; Kim, Hyang; Shin, Sang-Jin; Lee, Seung Yeol
- Issue Date
- Mar-2020
- Publisher
- BMC
- Keywords
- Tenocyte; Tonsil-derived mesenchymal stem cells; Bone marrow-derived mesenchymal stem cells; Design of experiments; Response surface methodology
- Citation
- JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH, v.15, no.1, pp.1 - 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
- Volume
- 15
- Number
- 1
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/146105
- DOI
- 10.1186/s13018-020-01623-8
- ISSN
- 1749-799X
- Abstract
- Background
In order to optimize the tenogenic differentiation of mesenchymal stem cells (MSCs), researchers should consider various factors. However, this requires testing numerous experimental settings, which is costly and time-consuming. We aimed to assess the differential effects of transforming growth factor beta-3 (TGF-β3) on the tenogenesis of tonsil-derived MSCs (T-MSCs) and bone marrow-derived MSCs (BM-MSCs) using response surface methodology (RSM).
Methods
Bone marrow and tonsillar tissue were collected from four patients; mononuclear cells were separated and treated with 5 or 10 ng/mL of TGF-β3. A full factorial experimental design with a categorical factor of 0 was employed to study the effect of tension based on T-MSCs. Eighty-four trials were fitted with RSM and then used to obtain mathematical prediction models.
Results
Exposure of T-MSCs and BM-MSCs to TGF-β3 increased the expression of scleraxis (SCX), tenomodulin (TNMD), decorin, collagen I, and tenascin C. Expression of most of these factors reached a maximum after 2–3 days of treatment. The model predicted that the values of the tenocyte lineage-related factors assessed would be significantly increased at 2.5 days of culture with 2.7 ng/mL of TGF-β3 for T-MSCs and at 2.3 days of culture regardless of TGF-β3 concentration for BM-MSCs.
Conclusions
This study demonstrated that the RSM prediction of the culture time necessary for the tenogenic differentiation of T-MSCs and BM-MSCs under TGF-β3 stimulation was similar to the experimentally determined time of peak expression of tenocyte-related mRNAs, suggesting the potential of using the RSM approach for optimization of the culture protocol for tenogenesis of MSCs.
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