Enhanced delivery of protein fused to cell penetrating peptides to mammalian cellsopen access
- Authors
- Moon, Jung-Il; Han, Min-Joon; Yu, Shin-Hye; Lee, Eun-Hye; Kim, Sang-Mi; Han, Kyuboem; Park, Chang-Hwan; Kim, Chun-Hyung
- Issue Date
- May-2019
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Keywords
- Amodiaquine (AQ); Cell Penetrating Peptide (CPP); Differentiation; Polylysine (9K); Reprogramming
- Citation
- BMB REPORTS, v.52, no.5, pp.324 - 329
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BMB REPORTS
- Volume
- 52
- Number
- 5
- Start Page
- 324
- End Page
- 329
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/147913
- DOI
- 10.5483/BMBRep.2019.52.5.195
- ISSN
- 1976-6696
- Abstract
- Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols.
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