Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
- Authors
- Lee, Yong Seung; Jung, Woon Yong; Heo, Hyejung; Park, Min Geun; Oh, Seung-Hun; Park, Byong-Gon; Kim, Soonhag
- Issue Date
- Mar-2018
- Publisher
- AMER CHEMICAL SOC
- Keywords
- ultrasound; exosome; fibroblast; cellular reprogramming; chromatin remodeling; neural progenitor
- Citation
- ACS NANO, v.12, no.3, pp.2531 - 2538
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACS NANO
- Volume
- 12
- Number
- 3
- Start Page
- 2531
- End Page
- 2538
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/150354
- DOI
- 10.1021/acsnano.7b08297
- ISSN
- 1936-0851
- Abstract
- Exosomes, naturally secreted nanoparticles, have been introduced as vehicles for horizontal transfer of genetic material. We induced autologous exosomes containing a cocktail of reprogramming factors ("reprosomes") to convert fibroblasts into neural progenitor cells (NPCs). The fibroblasts were treated with ultrasound and subsequently cultured in neural stem cell medium for 1 day to induce the release of reprosomes composed of reprogramming factors associated with chromatin remodeling and neural lineage-specific factors. After being treated with reprosomes, fibroblasts were converted into NPCs (rNPCs) with great efficiency via activation of chromatin remodeling, so quickly that only 5 days were required for the formation of 1500 spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal and proliferative properties for several weeks and successfully differentiated into neurons, astrocytes, and oligodendrocytes in vitro and in vivo. Reprosome-mediated cellular reprogramming is simple, safe, and efficient to produce autologous stem cells for clinical application.
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