Rapid preparation and single-cell analysis of concentrated blood smears using a high-throughput separator and a microfabricated grid film
- Authors
- You, Dongwon; Oh, Sein; Kim, Byeongyeon; Hahn, Young Ki; Choi, Sung young
- Issue Date
- Jul-2017
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Blood smears; Cytochemical analysis; Deterministic migration; Microfabricated grid film; Microfluidics.
- Citation
- JOURNAL OF CHROMATOGRAPHY A, v.1507, pp.141 - 148
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CHROMATOGRAPHY A
- Volume
- 1507
- Start Page
- 141
- End Page
- 148
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/151957
- DOI
- 10.1016/j.chroma.2017.05.047
- ISSN
- 0021-9673
- Abstract
- Cytological examination of peripheral white blood cells inhomogeneously distributed on a blood smear is currently limited by the low abundance and random sampling of the target cells. To address the challenges, we present a new approach to prepare and analyze concentrated blood smears by rapidly enriching white blood cells up to 32-fold with 92% recovery on average at a high throughput (1mL/min) using a deterministic migration-based separator and by systematically analyzing a large number of the cells distributed over a blood slide using a microfabricated grid film. We anticipate that our approach will improve the clinical utility of blood smear tests, while offering the capability to detect rare cell populations.
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