Genome-scale analysis of Acetobacterium bakii reveals the cold adaptation of psychrotolerant acetogens by post-transcriptional regulationopen access
- Authors
- Shin, Jongoh; Song, Yoseb; Jin, Sangrak; Lee, Jung-Kul; Kim, Dong Rip; Kim, Sun Chang; Cho, Suhyung; Cho, Byung-Kwan
- Issue Date
- Dec-2018
- Publisher
- COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
- Keywords
- post-transcriptional regulation; psychrotolerant acetogen; Acetobacterium bakii; cold-adaptive acetogenesis
- Citation
- RNA, v.24, no.12, pp.1839 - 1855
- Indexed
- SCIE
SCOPUS
- Journal Title
- RNA
- Volume
- 24
- Number
- 12
- Start Page
- 1839
- End Page
- 1855
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/15235
- DOI
- 10.1261/rna.068239.118
- ISSN
- 1355-8382
- Abstract
- Acetogens synthesize acetyl-CoA via CO₂ or CO fixation, producing organic compounds. Despite their ecological and industrial importance, their transcriptional and post-transcriptional regulation has not been systematically studied. With completion of the genome sequence of Acetobacterium bakii (4.28-Mb), we measured changes in the transcriptome of this psychrotolerant acetogen in response to temperature variations under autotrophic and heterotrophic growth conditions. Unexpectedly, acetogenesis genes were highly up-regulated at low temperatures under heterotrophic, as well as autotrophic, growth conditions. To mechanistically understand the transcriptional regulation of acetogenesis genes via changes in RNA secondary structures of 5'-untranslated regions (5'-UTR), the primary transcriptome was experimentally determined, and 1379 transcription start sites (TSS) and 1100 5'-UTR were found. Interestingly, acetogenesis genes contained longer 5'-UTR with lower RNA-folding free energy than other genes, revealing that the 5'-UTRs control the RNA abundance of the acetogenesis genes under low temperature conditions. Our findings suggest that post-transcriptional regulation via RNA conformational changes of 5'-UTRs is necessary for cold-adaptive acetogenesis.
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