Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regenerationopen access
- Authors
- Yoo, Chae-Kyung; Jeon, Jae-Yun; Kim, You-Jin; Kim, Seong-Gon; Hwang, Kyung Gyun
- Issue Date
- Mar-2016
- Publisher
- 대한악안면성형재건외과학회
- Keywords
- Bone regeneration; Cell adhesion; Cell proliferation; Membrane; Osteoblasts; Silk fibroin
- Citation
- Maxillofacial Plastic and Reconstructive Surgery, v.38, pp.1 - 6
- Indexed
- SCOPUS
KCI
- Journal Title
- Maxillofacial Plastic and Reconstructive Surgery
- Volume
- 38
- Start Page
- 1
- End Page
- 6
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/154938
- DOI
- 10.1186/s40902-016-0062-4
- ISSN
- 2288-8101
- Abstract
- Background: The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane forguided bone regeneration (GBR).
Methods: Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cellscultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, theattachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy(SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay,and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining.
Results: Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-dayobservation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (controlgroup) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly differentbetween the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane groupthan in the control group at 7 days (P < 0.05).
Conclusions: These results suggest that silk fibroin is a biocompatible material that could be used as a suitablealternative barrier membrane for GBR.
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