Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals
- Authors
- Chang, Mi-Yoon; Oh, Boram; Rhee, Yong-Hee; Lee, Sang-Hun
- Issue Date
- Nov-2015
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Human embryonic stem cell; Survival; Maintenance; Doxycycline
- Citation
- STEM CELL RESEARCH, v.15, no.3, pp.608 - 613
- Indexed
- SCIE
SCOPUS
- Journal Title
- STEM CELL RESEARCH
- Volume
- 15
- Number
- 3
- Start Page
- 608
- End Page
- 613
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/156013
- DOI
- 10.1016/j.scr.2015.10.007
- ISSN
- 1873-5061
- Abstract
- Culturing human embryonic stem and induced pluripotent stem cells (hESCs/iPSCs) is one of the most costly and labor-intensive tissue cultures, as media containing expensive factors/cytokines should be changed every day to maintain and propagate undifferentiated hESCs/iPSCs in vitro. We recently reported that doxycycline, an antibacterial agent, had dramatic effects on hESC/iPSC survival and promoted self-renewal. In this study, we extended the effects of doxycycline to a more practical issue to save cost and labor in hESC/iPSC cultures. Regardless of cultured cell conditions, hESCs/iPSCs in doxycycline-supplemented media were viable and proliferating for at least 3 days without media change, while none or few viable cells were detected in the absence of doxycycline in the same conditions. Thus, hESCs/iPSCs supplemented with doxycycline can be cultured for a long period of time with media changes at 3-day intervals without altering their self-renewal and pluripotent properties, indicating that doxycycline supplementation can reduce the frequency of media changes and the amount of media required by 1/3. These findings strongly encourage the use of doxycycline to save cost and labor in culturing hESCs/iPSCs.
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