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Eugenol Inhibits the GABA(A) Current in Trigeminal Ganglion Neuronsopen access

Authors
Lee, Sang HoonMoon, Jee YounJung, Sung JunKang, Jin GuChoi, Seung PyoJang, Jun Ho
Issue Date
Jan-2015
Publisher
PUBLIC LIBRARY SCIENCE
Citation
PLOS ONE, v.10, no.1, pp.1 - 12
Indexed
SCIE
SCOPUS
Journal Title
PLOS ONE
Volume
10
Number
1
Start Page
1
End Page
12
URI
https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/158189
DOI
10.1371/journal.pone.0117316
ISSN
1932-6203
Abstract
Eugenol has sedative, antioxidant, anti-inflammatory, and analgesic effects, but also serves as an irritant through the regulation of a different set of ion channels. Activation of gamma aminobutyric acid (GABA) receptors on sensory neurons leads to the stabilization of neuronal excitability but contributes to formalin-induced inflammatory pain. In this study, we examined the effect of eugenol on the GABA-induced current in rat trigeminal ganglia (TG) neurons and in human embryonic kidney (HEK) 293 cells expressing the GABA(A) receptor alpha 1 beta 2 gamma 2 subtype using the whole-cell patch clamp technique. RT-PCR and Western blot analysis were used to confirm the expression of GABA(A) receptor gamma 2 subunit mRNA and protein in the TG and hippocampus. Eugenol decreased the amplitude ratio of the GABA-induced current to 27.5 +/- 3.2% (p < 0.05) in TG neurons, which recovered after a 3-min washout. In HEK 293 cells expressing the alpha 1 beta 2.2 subtype, eugenol inhibited GABA-induced currents in a dose-dependent manner. Application of eugenol also decreased the GABA response in the presence of a G-protein blocker. Eugenol pretreatment with different concentrations of GABA resulted in similar inhibition of the GABA-induced current in a noncompetitive manner. In conclusion, eugenol inhibits the GABA-induced current in TG neurons and HEK 293 cells expressing the GABA(A) receptor in a reversible, dose-dependent, and non-competitive manner, but not via the G-protein pathway. We suggest that the GABA(A) receptor could be a molecular target for eugenol in the modulation of nociceptive information.
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