Clostridium difficile infection aggravates colitis in interleukin 10-deficient mice
- Authors
- Kim, Mi Na; Koh, Seong-Joon; Kim, Jung Mogg; Im, Jong Pil; Jung, Hyun Chae; Kim, Joo Sung
- Issue Date
- Dec-2014
- Publisher
- BAISHIDENG PUBLISHING GROUP INC
- Keywords
- Clostridium difficile; Inflammatory bowel disease; Interleukin 10-deficient mice; Interferon-gamma; Colitis
- Citation
- WORLD JOURNAL OF GASTROENTEROLOGY, v.20, no.45, pp.17084 - 17091
- Indexed
- SCIE
SCOPUS
- Journal Title
- WORLD JOURNAL OF GASTROENTEROLOGY
- Volume
- 20
- Number
- 45
- Start Page
- 17084
- End Page
- 17091
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/158498
- DOI
- 10.3748/wjg.v20.i45.17084
- ISSN
- 1007-9327
- Abstract
- AIM: To investigate the effect of Clostridium difficile (C. difficile) infection in an interleukin 10-deficient (IL-10(-/-)) mouse model of inflammatory bowel disease. METHODS: Bone marrow-derived dendritic cells isolated from wild type (WT) and IL-10(-/-) mice were stimulated for 4 h with C. difficile toxin A (200 mu g/mL), and gene expression of interferon (IFN)-gamma, IL-12 and IL-23 was determined by real-time reverse transcription polymerase chain reaction. WT and IL-10(-/-) mice (n = 20 each) were exposed to an antibiotic cocktail for three days and then were injected with clindamycin (i.p.). Mice (n = 10 WT, 10 IL-10(-/-)) were then challenged with oral administration of C. difficile (1 x 10(5) colony forming units of strain VPI 10463). Animals were monitored daily for 7 d for signs of colitis. Colonic tissue samples were evaluated for cytokine gene expression and histopathologic analysis. RESULTS: C. difficile toxin A treatment induced IFN-gamma gene expression to a level that was significantly higher in BDMCs from IL-10(-/-) compared to those from WT mice (P < 0.05). However, expression of IL-12 and IL-23 was not different among the groups. Following C. difficile administration, mice developed diarrhea and lost weight within 2-3 d. Weight loss was significantly greater in IL-10(-/-) compared to WT mice (P < 0.05). C. difficile infection induced histopathologic features typical of colitis in both IL-10(-/-) and WT mice. The histopathologic severity score was significantly higher in the IL-10(-/)- than in WT mice (mean +/- standard error; 5.50 +/- 0.53 vs 2.44 +/- 0.46; P < 0.05). This was accompanied by a significantly greater increase in IFN-gamma gene expression in colonic tissues from IL-10(-/-) than from WT mice challenged with C. difficile (P < 0.05). CONCLUSION: These results indicate that colitis is more severe after C. difficile infection in IL-10(-/-) mice, and that IFN-gamma expression is involved in this process. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.
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