Enrichment of cells with TALEN-induced mutations using surrogate reporters
- Authors
- Kim, Young-Hoon; Ramakrishna, Suresh; Kim, Hyongbum; Kim, Jin-Soo
- Issue Date
- Aug-2014
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Transcription activator-like effector nucleases; Enrichment; Mutant cells; Flow cytometry; Magnetic separation; Hygromycin B
- Citation
- METHODS, v.69, no.1, pp.108 - 117
- Indexed
- SCIE
SCOPUS
- Journal Title
- METHODS
- Volume
- 69
- Number
- 1
- Start Page
- 108
- End Page
- 117
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/159441
- DOI
- 10.1016/j.ymeth.2014.04.012
- ISSN
- 1046-2023
- Abstract
- Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection.
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