Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model
- Authors
- Lee, Jangwook; Cha, Min-Ji; Lim, Kwang Suk; Kim, Jang-Kyung; Lee, Sang-Kyung; Kim, Yong-Hee; Hwang, Ki-Chul; Lee, Kuen Yong
- Issue Date
- Nov-2013
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Heat shock protein; microsphere/hydrogel hybrid system; myocardial infarction
- Citation
- JOURNAL OF DRUG TARGETING, v.21, no.9, pp.822 - 829
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF DRUG TARGETING
- Volume
- 21
- Number
- 9
- Start Page
- 822
- End Page
- 829
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/161555
- DOI
- 10.3109/1061186X.2013.829072
- ISSN
- 1061-186X
- Abstract
- Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(D,L-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.
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