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곡류 중 아플라톡신 B1 신속 검출용 간이진단키트의 개발Development of Rapid Detection Kit for Aflatoxin B1 in Cereals

Other Titles
Development of Rapid Detection Kit for Aflatoxin B1 in Cereals
Authors
최선미김기영이상대문지혜엄애선
Issue Date
Aug-2013
Publisher
한국산업식품공학회
Keywords
Aflatoxin B1; Rapid detection kit; Cereals; Corn
Citation
산업식품공학, v.17, no.3, pp.271 - 275
Indexed
KCI
Journal Title
산업식품공학
Volume
17
Number
3
Start Page
271
End Page
275
URI
https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162181
DOI
10.13050/foodengprog.2013.17.3.271
ISSN
1226-4768
Abstract
The aim of this study is to develop the one dot rapid detection kit that meets the limit of detection (LOD) of KFDA (10 μg/kg) for Aflatoxin B1 (AFB1). The fabrication process of the one dot rapid detection kit, such as gold nanoparticle and AFB1-BSA conjugation, and AFB1-polyclonal antibody concentration was optimized. Fabricated rapid detection kits were tested with the standard AFB1 solutions and the solutions extracted from contaminated maize samples. The LOD in the standard AFB1 solution and extracted AFB1 solution was 5 μg/kg for both. This result is lower than the LOD of KFDA in food (10 μg/kg) and satisfies the LOD of EU (2-12 μg/kg) and Japan (10 μg/kg) in food.
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서울 생활과학대학 > 서울 식품영양학과 > 1. Journal Articles

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COLLEGE OF HUMAN ECOLOGY (DEPARTMENT OF FOOD & NUTRITION)
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