The phenyl-thiophenyl propenone RK-I-123 reduces the levels of reactive oxygen species and suppresses the activation of NF-kappa B and AP-1 and IL-8 expression in Helicobacter pylori-infected gastric epithelial AGS cells
- Authors
- Jang, Sung Hee; Cho, Soonok; Lee, Eung-Seok; Kim, Jung Mogg; Kim, Hyeyoung
- Issue Date
- Jul-2013
- Publisher
- SPRINGER BASEL AG
- Keywords
- Helicobacter pylori; RK-I-123; IL-8; NF-kappa B; Gastric epithelial cells
- Citation
- INFLAMMATION RESEARCH, v.62, no.7, pp.689 - 696
- Indexed
- SCIE
SCOPUS
- Journal Title
- INFLAMMATION RESEARCH
- Volume
- 62
- Number
- 7
- Start Page
- 689
- End Page
- 696
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162401
- DOI
- 10.1007/s00011-013-0621-4
- ISSN
- 1023-3830
- Abstract
- To investigate whether the phenyl-thiophenyl propenone RK-I-123 suppresses interleukin-8 (IL-8) expression and activation of mitogen-activated protein kinases (MAPKs) and transcription factors (nuclear factor-kappa B [NF-kappa B] and activator protein-1 [AP-1]) by reducing reactive oxygen species (ROS) levels in Helicobacter pylori-infected gastric epithelial cells. Helicobacter pylori in Korean isolates, human gastric epithelial AGS cells. AGS cells pretreated with or without RK-I-123 were cultured in the presence of H. pylori at a bacterium/cell ratio of 300:1. Reactive oxygen species and IL-8 levels were determined by dichlorofluorescein fluorescence and enzyme-linked immunosorbent assay. The IL-8 mRNA expression was analyzed by the real-time reverse transcription-polymerase chain reaction (RT-PCR). The MAPK and I kappa B alpha levels were determined by western blotting. The activation of NF-kappa B and AP-1 was determined by the electrophoretic mobility shift assay. Helicobacter pylori induced an increase in ROS and IL-8 expression and activation of MAPKs and transcription factors (NF-kappa B and AP-1) together with the degradation of I kappa B alpha in AGS cells, all of which were inhibited by RK-I-123. The RK-I-123 suppressed the H. pylori-induced IL-8 expression and activation of MAPKs, NF-kappa B, and AP-1 by reducing ROS levels in AGS cells. The RK-I-123 may be a potential candidate for the treatment of H. pylori-induced gastric inflammation.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의과대학 > 서울 미생물학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162401)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.