Silaffin Peptides as a Novel Signal Enhancer for Gravimetric Biosensorsopen access
- Authors
- Dong Hyun Nam; Lee, Jeong-O; Sang, Byoung-In; Won, Keehoon; Kim, Yong Hwan
- Issue Date
- May-2013
- Publisher
- HUMANA PRESS INC
- Keywords
- Silaffin peptides; Biomimetic silica formation; R5 peptide; Quartz crystal microbalances; Signal enhancer
- Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.170, no.1, pp.25 - 31
- Indexed
- SCIE
SCOPUS
- Journal Title
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Volume
- 170
- Number
- 1
- Start Page
- 25
- End Page
- 31
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162867
- DOI
- 10.1007/s12010-013-0161-y
- ISSN
- 0273-2289
- Abstract
- Application of biomimetic silica formation to gravimetric biosensors has been conducted for the first time. As a model system, silaffin peptides fused with green fluorescent protein (GFP) were immobilized on a gold quartz crystal resonator for quartz crystal microbalances using a self-assembled monolayer. When a solution of silicic acid was supplied, silica particles were successfully deposited on the Au surface, resulting in a significant change in resonance frequency (i.e., signal enhancement) with the silaffin-GFP. However, frequency was not altered when bare GFP was used as a control. The novel peptide enhancer is advantageous because it can be readily and quantitatively conjugated with sensing proteins using recombinant DNA technology. As a proof of concept, this study shows that the silaffin domains can be employed as a novel and efficient biomolecular signal enhancer for gravimetric biosensors.
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