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뉴트라비딘 검출용 간이 진단키트의 성능향상을 위한 기본 플랫폼 선정The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit
- Other Titles
- The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit
- Issue Date
- Dec-2012
- Publisher
- 농업과학연구소
- Keywords
- Rapid detection kit; Nitrocellulose membrane; Avidin-biotin interaction
- Citation
- Korean Journal of Agricultural Science, v.39, no.4, pp 613 - 618
- Pages
- 6
- Indexed
- KCICANDI
- Journal Title
- Korean Journal of Agricultural Science
- Volume
- 39
- Number
- 4
- Start Page
- 613
- End Page
- 618
- ISSN
- 2466-2402
2466-2410
- Abstract
- This study was performed to optimize the basic platform of a lateral flow immunoassay. Improvement of the limit of detection (LOD) was evaluated according to the width of a nitrocellulose membrane with varying concentrations of analyte. The analyte, neutravidin was detected based on the avidin-biotin interaction. The antibody-Au nanoparticle conjugation was mostly stabled in a PBS buffer of pH 7.3. The optimal widths of a nitrocellulose membrane were 4and 6 mm considering the sample flow rate and signal strength of the test line on the membrane. The LOD of neutravidin was 0.001 mg/ml in the optimum conditions.
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Collections - 서울 생활과학대학 > 서울 식품영양학과 > 1. Journal Articles
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