뉴트라비딘 검출용 간이 진단키트의 성능향상을 위한 기본 플랫폼 선정The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit
- Other Titles
- The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit
- Authors
- 최선미; 김기영; 엄애선; 문지혜; 박샛별; 이상대; 김혁주
- Issue Date
- Dec-2012
- Publisher
- 충남대학교 농업과학연구소
- Keywords
- Rapid detection kit; Nitrocellulose membrane; Avidin-biotin interaction
- Citation
- Korean Journal of Agricultural Science, v.39, no.4, pp.613 - 618
- Indexed
- KCI
OTHER
- Journal Title
- Korean Journal of Agricultural Science
- Volume
- 39
- Number
- 4
- Start Page
- 613
- End Page
- 618
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/163948
- DOI
- 10.7744/cnujas.2012.39.4.613
- ISSN
- 2466-2402
- Abstract
- This study was performed to optimize the basic platform of a lateral flow immunoassay. Improvement of the limit of detection (LOD) was evaluated according to the width of a nitrocellulose membrane with varying concentrations of analyte. The analyte, neutravidin was detected based on the avidin-biotin interaction. The antibody-Au nanoparticle conjugation was mostly stabled in a PBS buffer of pH 7.3. The optimal widths of a nitrocellulose membrane were 4and 6 mm considering the sample flow rate and signal strength of the test line on the membrane. The LOD of neutravidin was 0.001 mg/ml in the optimum conditions.
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