Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes
- Authors
- Bui, Hong-Thuy; Kwon, Deug-Nam; Kang, Min-Hui; Oh, Mi-Hye; Park, Mi-Ryung; Park, Woo-Jin; Paik, Seung-Sam; Nguyen Van Thuan; Kim, Jin-Hoi
- Issue Date
- Dec-2012
- Publisher
- COMPANY BIOLOGISTS LTD
- Keywords
- Epigenetic reprogramming; Histone methylation; Nuclear transfer; Reprogrammed cells; Oocyte extract
- Citation
- DEVELOPMENT, v.139, no.23, pp.4330 - 4340
- Indexed
- SCIE
SCOPUS
- Journal Title
- DEVELOPMENT
- Volume
- 139
- Number
- 23
- Start Page
- 4330
- End Page
- 4340
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164048
- DOI
- 10.1242/dev.086116
- ISSN
- 0950-1991
- Abstract
- Genomic reprogramming factors in the cytoplasm of germinal vesicle (GV) stage oocytes have been shown to improve the efficiency of producing cloned mouse offspring through the exposure of nuclei to a GV cytoplasmic extract prior to somatic cell nuclear transfer (SCNT) to enucleated oocytes. Here, we developed an extract of GV stage pig oocytes (GVcyto-extract) to investigate epigenetic reprogramming events in treated somatic cell nuclei. This extract induced differentiation-associated changes in fibroblasts, resulting in cells that exhibit pluripotent stem cell-like characteristics and that redifferentiate into three primary germ cell layers both in vivo and in vitro. The GVcyto-extract treatment induced large numbers of high-quality SCNT-generated blastocysts, with methylation and acetylation of H3-K9 and expression of Oct4 and Nanog at levels similar to in vitro fertilized embryos. Thus, GVcyto-extract could elicit differentiation plasticity in treated fibroblasts, and SCNT-mediated reprogramming reset the epigenetic state in treated cells more efficiently than in untreated cells. In summary, we provide evidence for the generation of stem-like cells from differentiated somatic cells by treatment with porcine GVcyto-extract.
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