Butyl paraben-induced changes in DNA methylation in rat epididymal spermatozoa
- Authors
- Park, Chan Jin; Nah, Won Heum; Lee, Jae-Eun; Yoon Sik; Gye, Myung Chan
- Issue Date
- May-2012
- Publisher
- WILEY-BLACKWELL
- Keywords
- Butyl paraben; DNA methylation; epididymal spermatozoa; rat
- Citation
- ANDROLOGIA, v.44, pp.187 - 193
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANDROLOGIA
- Volume
- 44
- Start Page
- 187
- End Page
- 193
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/165714
- DOI
- 10.1111/j.1439-0272.2011.01162.x
- ISSN
- 0303-4569
- Abstract
- Parabens have been shown to affect male rodent reproductive parameters, including testosterone levels and sperm production. In this study, we examined the effect of long-term exposure to butyl paraben (BP) on rat epididymal sperm DNA methylation. Adult male rats were exposed to BP (0, 10, 100 and 1000 mg kg-1 per day) according to OECD TG407 for a repeated 28-day oral toxicity study. Sperm DNA methylation was examined by differential display random amplification of polymorphic DNA (RAPD) following methylation-specific restriction digestion of DNA. Among the 57 RAPD amplicons, six were methylation specific. Of these, five amplicons increased by 1.4- to 3.8-fold in epididymal sperm DNA at testing dose of BP. This indicates that BP can cause DNA hypermethylation in germ cells from the mitotic through post-meiotic stage in adult rat testes. To our knowledge, this is the first report on the epigenetic modification of sperm DNA by parabens.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 자연과학대학 > 서울 생명과학과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.