Experimental validation of the solvent-gradient simulated moving bed process for optimal separation of phenylalanine and tryptophan
- Authors
- Nam, Hee-Geun; Jo, Se-Hee; Park, Chanhun; Mun, Sungyong
- Issue Date
- Mar-2012
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Phenylalanine; Tryptophan; Poly-4-vinylpyridine (PVP); Amino acid separation; Simulated moving bed
- Citation
- PROCESS BIOCHEMISTRY, v.47, no.3, pp.401 - 409
- Indexed
- SCIE
SCOPUS
- Journal Title
- PROCESS BIOCHEMISTRY
- Volume
- 47
- Number
- 3
- Start Page
- 401
- End Page
- 409
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166123
- DOI
- 10.1016/j.procbio.2011.11.011
- ISSN
- 1359-5113
- Abstract
- A solvent-gradient simulated moving bed (SG-SMB) process for separation of two useful amino acids, phenylalanine and tryptophan, has been studied in previous researches, which confirmed that the SG-SMB outperformed the corresponding isocratic SMB for the same separation task. However, all of the previous studies on the SG-SMB for separation of the two amino acids have been limited to process simulation and optimization, including some batch-chromatography tests. The experimental validation of such an SG-SMB process was attempted in this article. This task began by assembling the experimental unit of the SG-SMB process. Its operating conditions were determined from the SG-SMB optimization tool based on genetic algorithm, in which the mass-transfer parameters as well as the adsorption isotherm parameters of the feed components (two amino acids) were entered as the mathematical models expressed as a function of liquid-phase composition (i.e., modifier concentration in the liquid phase). Based on the determined operating conditions, the relevant SG-SMB experiment was conducted until a cyclic steady-state was reached. The assay of all the resultant product samples verified that the SG-SMB separation of interest was performed successfully as designed. The experimental data were also found to agree closely with the model predictions.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 공과대학 > 서울 화학공학과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.