Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry
- Authors
- Ahn, Jang-Hyuk; Jeong, Young-Su; Lee, Tae Geol; Kim, Young-Pil; Kim, Hak-Sung
- Issue Date
- Mar-2012
- Publisher
- KOREAN BIOCHIP SOCIETY-KBCS
- Keywords
- Aflatoxin; TOF-SIMS; Quantification; Multiplexed analysis
- Citation
- BIOCHIP JOURNAL, v.6, no.1, pp.34 - 40
- Indexed
- SCIE
SCOPUS
KCI
OTHER
- Journal Title
- BIOCHIP JOURNAL
- Volume
- 6
- Number
- 1
- Start Page
- 34
- End Page
- 40
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166164
- DOI
- 10.1007/s13206-012-6105-8
- ISSN
- 1976-0280
- Abstract
- A rapid and reliable analysis of toxins is prerequisite for food control and human healthcare. Here we demonstrate a simple and multiplexed assay of aflatoxins using time-of-flight secondary ion mass spectrometry (TOF-SIMS). By simply adsorbing either a single analyte or mixed ones onto a gold substrate, the corresponding secondary molecular ions ([M+H](+)) were clearly observed in a single mass spectrum. As a result of concentration-dependent peak intensity, quantitative and multiplexed analysis of different aflatoxin analogs from corns was accomplished with immunoaffinity column and TOF-SIMS analysis, which showed a good correlation with HPLC data. The detection sensitivity was estimated to be as low as 10 ng mL(-1) This approach presented here will find a wide application to detection of low-levels of toxins in a rapid and multiplexed way.
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