CNP-1 (ARRD-17), a Novel Substrate of Calcineurin, Is Critical for Modulation of Egg-Laying and Locomotion in Response to Food and Lysine Sensation in Caenorhabditis elegans
- Authors
- Jee, Changhoon; Choi, Tae-Woo; Kalichamy, Karunambigai; Yee, Jong Zin; Song, Hyun-Ok; Ji, Yon Ju; Lee, Jungsoo; Lee, Jin Il; L'Etoile, Noelle D.; Ahnn, Joohong; Lee, Sun-Kyung
- Issue Date
- Mar-2012
- Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- Keywords
- calcineurin; CNP-1; ARRD-17; ASK; ASH
- Citation
- JOURNAL OF MOLECULAR BIOLOGY, v.417, no.3, pp.165 - 178
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF MOLECULAR BIOLOGY
- Volume
- 417
- Number
- 3
- Start Page
- 165
- End Page
- 178
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166174
- DOI
- 10.1016/j.jmb.2012.01.012
- ISSN
- 0022-2836
- Abstract
- Calcineurin is a Ca2+/calmodulin-dependent protein phosphatase involved in calcium signaling pathways. In Caenorhabditis elegans, the loss of calcineurin activity causes pleiotropic defects including hyperadaptation of sensory neurons, hypersensation to thermal difference and hyper-egg-laying when worms are refed after starvation. In this study, we report on arrd-17 as calcineurin-interacting protein-1 (cnp-1), which is a novel molecular target of calcineurin. CNP-1 interacts with the catalytic domain of the C. elegans calcineurin A subunit, TAX-6, in a yeast two-hybrid assay and is dephosphorylated by TAX-6 in vitro. cnp-1 is expressed in ASK, ADL, ASH and ASJ sensory neurons as TAX-6. It acts downstream of tax-6 in regulation of locomotion and egg-laying after starvation, ASH sensory neuron adaptation and lysine chemotaxis, that is known to be mediated by ASK neurons. Altogether, our biochemical and genetic evidence indicates that CNP-1 is a direct target of calcineurin and required in stimulated egg-laying and locomotion after starvation, adaptation to hyperosmolarity and attraction to lysine, which is modulated by calcineurin. We suggest that the phosphorylation status of CNP-1 plays an important role in regulation of refed stimulating behaviors after starvation and attraction to amino acid, which provides valuable nutritious information.
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