Recombinant human erythropoietin reduces aggregation of mutant Cu/Zn-binding superoxide dismutase (SOD1) in NSC-34 cells
- Authors
- Cho, Goang-Won; Kim, Ga-Young; Baek, Soojeong; Kim, Heejaung; Kim, Taikon; Kim, Hee Jin; Kim, Seung Hyun
- Issue Date
- Oct-2011
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- Erythropoietin; ALS; SOD1; Aggregation
- Citation
- NEUROSCIENCE LETTERS, v.504, no.2, pp.107 - 111
- Indexed
- SCIE
SCOPUS
- Journal Title
- NEUROSCIENCE LETTERS
- Volume
- 504
- Number
- 2
- Start Page
- 107
- End Page
- 111
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167527
- DOI
- 10.1016/j.neulet.2011.09.008
- ISSN
- 0304-3940
- Abstract
- Human erythropoietin (hEPO) has multiple actions in non-hematopoietic tissues, including neurotrophic, anti-oxidant, anti-apoptotic, and anti-inflammatory effects. To examine the effect of EPO in an vitro model of amyotrophic lateral sclerosis (ALS), we stably overexpressed wild SOD1 and a mutant form. SOD1/G93A, in NSC-34 motoneuron-like cells. Transformants harboring the wild and mutant forms of SOD1 were selected by G418 selection and immunoblot analysis. RT-PCR analysis showed that cox-2 expression was increased in the NSC-34/mSOD1s, and MU assays and BrdU-ELISAs revealed reduced cell growth and proliferation in the NSC-34/mSOD1 cell line. Incubation with 5 or 10 IU/mL rhEPO increased the viability and decreased the cox-2 expression in the dNSC-34/mSOD1s cells. Immunocytochemical staining with anti-SOD1 antibody revealed the presence of aggregates of mSOD1 protein in dNSC-34/mSOD1 cells. Incubation with10 IU/mL rhEPO reduced the proportion of cells containing such aggregates. Our findings suggest that the anti-oxidant and anti-inflammatory effects of EPO increase the survival of NSC-34/mSOD1 cells and reduce aggregation of the mutant SOD1 protein.
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