Identification of Antigenic Proteins in Trichomonas vaginalisopen access
- Authors
- Lee, Hye-Yeon; Hyung, Sujin; Lee, Jong Woong; Kim, Juri; Shin, Myeong Heon; Ryu, Jae-Sook; Park, Soon-Jung
- Issue Date
- Mar-2011
- Publisher
- KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI
- Keywords
- Trichomonas vaginalis; antigenic protein; membrane protein; immunoscreening
- Citation
- KOREAN JOURNAL OF PARASITOLOGY, v.49, no.1, pp.79 - 83
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- KOREAN JOURNAL OF PARASITOLOGY
- Volume
- 49
- Number
- 1
- Start Page
- 79
- End Page
- 83
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/168885
- DOI
- 10.3347/kjp.2011.49.1.79
- ISSN
- 0023-4001
- Abstract
- Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T vaginalis. The T vaginalis expression library was constructed by cloning the cDNA derived from mRNA of T vaginalis into a phage lambda Uni-ZAP XR vector, and then used for immunoscreening with the anti-membrane proteins of T. vaginalis antibodies. The immunoreactive proteins identified included adhesion protein AP65-1, alpha-actinin, kinesin-associated protein, teneurin, and 2 independent hypothetical proteins. Immunofluorescence assays showed that AP65-1, one of the identified immunogenic clones, is prevalent in the whole body of T vaginalis. This study led us to identify T vaginalis proteins which may stimulate immune responses by human cells.
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