Preparation of Dexamethasone-Based Cationic Liposome and Its Application to Gene Delivery In Vitro
- Authors
- Kim, Tae-Hun; Yu, Gwang Sig; Choi, Hye; Shim, Young Jung; Lee, Minhyung; Choi, Joon Sig
- Issue Date
- Feb-2011
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Dendrimer; Dexamethasone; Cationic Liposome; Gene Transfection
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.11, no.2, pp.1799 - 1802
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 11
- Number
- 2
- Start Page
- 1799
- End Page
- 1802
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/169091
- DOI
- 10.1166/jnn.2011.3407
- ISSN
- 1533-4880
- Abstract
- In this study, dexamethasone was conjugated to PAMAM dendrimer (generation 0) and its gene transfection efficiency was investigated. To make a liposomal solution for gene delivery, DOPE was used as a fusogenic helper lipid. In gel retardation assay, PAMAM-dexamethasone conjugate (PAM-Dex)/DOPE liposome/DNA complex was completely retarded at 8:1 N/P (nitrogen/phosphate) ratio. The physicochemical characteristics are studied by measuring the average size distribution and zeta-potential values of the complexes. In vitro transfection assay showed that the PAM-Dex/DOPE liposome/DNA complex displayed higher gene delivery efficiency compared to PAMAM/DNA complex. In addition, PAM-Dex/DOPE liposome showed the lowest toxicity compared to PAMAM, PEI 25 kD and Lipofectamine. These results indicate that PAM-Dex/DOPE liposome has a potential to be used as an efficient gene carrier for gene therapy.
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