Antioxidant effect of lidocaine and procaine on reactive oxygen species-induced endothelial dysfunction in the rabbit abdominal aortaopen access
- Authors
- Jae, Myeong Lee; Jung, Kook Suh; Ji, Seon Jeong; Sang, Yun Cho; Dong, Won Kim
- Issue Date
- Aug-2010
- Publisher
- 대한마취통증의학회
- Keywords
- 3-amino-1,2,4-triazole; Catalase; Endothelium; Lidocaine; Procaine; Reactive oxygen species
- Citation
- Korean Journal of Anesthesiology, v.59, no.2, pp.104 - 110
- Indexed
- SCOPUS
KCI
- Journal Title
- Korean Journal of Anesthesiology
- Volume
- 59
- Number
- 2
- Start Page
- 104
- End Page
- 110
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/174326
- DOI
- 10.4097/kjae.2010.59.2.104
- ISSN
- 2005-6419
- Abstract
- Background: Reactive oxygen species (ROS) induce lipid peroxidation and tissue damage in the endothelium. We tested the antioxidant effect of lidocaine and procaine on ROS-induced endothelial damage in the rabbit aorta. Methods: Aortic rings isolated from rabbits were suspended in an organ bath filled with Krebs-Henseleit (K-H) solution bubbled with 5% CO2 and 95% O 2 at 37.5°C. After precontraction with phenylephrine (PE, 10 -6 M), changes in tension were recorded following a cumulative administration of acetylcholine (ACh 3 × 10-8 to 10 -6 M). Differences were measured as percentages of ACh-induced relaxation of aortic rings before and after exposure to ROS as generated by electrolysis of the K-H solution. The aortic rings were pretreated with lidocaine or procaine (10-5 M to 3 × 10-3 M) to compare their effects, as well as ROS scavengers, catalase, mannitol, sodium salicylate, and deferoxamine, and a catalase inhibitor, 3-amino-1,2,4-triazole (3AT). Results: Lidocaine and procaine dose-dependently maintained endothelium-dependent relaxation induced by ACh despite ROS activity (P < 0.05 vs control value). The 3AT pretreated procaine (3 × 10-3 M) group decreased more significantly than the un-pretreated procaine group (P < 0.05). Conclusions: These findings suggest that lidocaine and procaine dose-dependently preserve endothelium-dependent vasorelaxation against ROS attack, potentially via hydrogen peroxide scavenging. Copyright © Korean Society of Anesthesiologists, 2010.
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