Optimal design and experimental validation of a modified four-zone SMB process for the separation of a ternary amino acid mixture
- Authors
- Jo, Se-Hee; Nam, Hee-Geun; Mun, Sungyong
- Issue Date
- Aug-2010
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Modified four-zone SMB; Ternary separation; Experimental validation; Process optimization; Amino acid mixture; Model simulation
- Citation
- PROCESS BIOCHEMISTRY, v.45, no.8, pp.1288 - 1298
- Indexed
- SCIE
SCOPUS
- Journal Title
- PROCESS BIOCHEMISTRY
- Volume
- 45
- Number
- 8
- Start Page
- 1288
- End Page
- 1298
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/174343
- DOI
- 10.1016/j.procbio.2010.04.016
- ISSN
- 1359-5113
- Abstract
- For a standard four-zone simulated moving bed (SMB) chromatography, several process modifications have been made in previous studies such that its application scope could be extended to a ternary separation. One of the effective modifications reported was to (1) replace its closed-loop configuration with an open-loop configuration and (2) utilize the extract port for collecting both the intermediate-affinity and the highest-affinity components in regular sequence in every switching period. Most of previous researches on such a modified four-zone SMB (MF-SMB) have been limited to process simulation and optimization. The experimental validation of the MF-SMB process with linear isotherms was attempted in this article for the first time using a ternary amino acid mixture as a model system. First, the intrinsic parameters of three amino acids were estimated from a series of multiple-frontal experiments. The estimated parameter values were then used in the stage of the MF-SMB optimization, which was assisted by an up-to-date genetic algorithm. Based on the optimized conditions, the MF-SMB experiment was conducted and the assay results for product samples verified the attainment of a ternary separation. The experimental purities and concentrations were also found to agree closely with the model predictions.
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