Effects of Endocrine Disruptors on Bombina orientalis P450 Aromatase Activity
- Authors
- Lee, Kyung-min; Yang, Wonseok; Rhee, Jae-Sung; Hwang, Dae-Sik; Park, Chan Jin; Gye, Myung Chan; Lee, Jae-Seong; Shin, Incheol
- Issue Date
- Apr-2010
- Publisher
- ZOOLOGICAL SOC JAPAN
- Keywords
- amphibians; aromatase; bisphenol-A; steroid; steroidogenesis
- Citation
- ZOOLOGICAL SCIENCE, v.27, no.4, pp.338 - 343
- Indexed
- SCIE
SCOPUS
- Journal Title
- ZOOLOGICAL SCIENCE
- Volume
- 27
- Number
- 4
- Start Page
- 338
- End Page
- 343
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/175155
- DOI
- 10.2108/zsj.27.338
- ISSN
- 0289-0003
- Abstract
- To assess the effects of endocrine-disrupting chemicals on the expression and activity of aromatase in the gonads of Bombina orientalis, a common amphibian, we intraperitoneally injected nonylphenol or bisphenol-A and then examined aromatase mRNA levels by RT-PCR as well as aromatase enzymatic activity by tritiated water release assays. To design primers for the RT-PCR, we cloned the B. orientalis aromatase gene using RT-PCR and degenerate primers. The full-length cDNA was obtained by 5'- and 3'-RACE PCR. The complete sequence of the B. orientalis aromatase gene revealed an open reading frame of 1500 bp encoding a deduced protein of 500 amino acids. Semi-quantitative RT-PCR indicated that nonylphenol or bisphenol-A injection did not significantly affect the expression of B. orientalis aromatase mRNA. However, a 48-hr treatment with nonyphenol or bisphenol A reduced aromatase activity to 47% and 32% of the control, respectively. These results suggest that endocrine disruptors can effectively modulate the activity of B. orientalis aromatase without affecting its mRNA levels.
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