A guanidinylated bioreducible polymer with high nuclear localization ability for gene delivery systemsopen access
- Authors
- Kim, Tae-il; Lee, Minhyung; Kim, Sung Wan
- Issue Date
- Mar-2010
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Bioreducible polymer; Gene transfer; Guanidinylation; Cytotoxicity; Nuclear localization ability
- Citation
- BIOMATERIALS, v.31, no.7, pp.1798 - 1804
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMATERIALS
- Volume
- 31
- Number
- 7
- Start Page
- 1798
- End Page
- 1804
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/175364
- DOI
- 10.1016/j.biomaterials.2009.10.034
- ISSN
- 0142-9612
- Abstract
- Guanidinylated bioreducible polymer (GBP) was developed for gene delivery systems utilizing cellular penetrating ability of guanidine groups. GBP could retard pDNA from a weight ratio of 5 completely in agarose gel electrophoresis but pDNA was released from GBP polyplexes even at a weight ratio of 20 in reducing condition (2.5 mm DTT) due to their biodegradation. GBP also could construct 200 nm-sized and positively charged (similar to 30 mV) polyplex nanoparticles with pDNA. The cytotoxicity of GBP was found to be minimal and GBP showed about 8 folds improved transfection efficiency than a scaffold polymer, poly(cystaminebisacrylamide-diaminohexane) (poly(CBA-DAH)) and even higher transfection efficiency than PEI25k in mammalian cell lines. Its high cellular uptake efficiency (96.1%) and strong nuclear localization ability for pDNA delivery due to the structural advantage of bioreducible polymer and guanidine groups were also identified, suggesting GBP is a promising candidate for efficient gene delivery systems.
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