Identification of proteomic biomarkers of preeclampsia in amniotic fluid using SELDI-TOF mass spectrometry
- Authors
- Park, Joong Shin; Oh, Kyoung-Jin; Norwitz, Errol R.; Han, Joong-Soo; Chol, Hye-Jin; Seong, Hyo Suk; Kang, Yoon Dan; Park, Chan-Wook; Kim, Byoung Jae; Jun, Jong Kwan; Syn, Hee Chul
- Issue Date
- May-2008
- Publisher
- SAGE PUBLICATIONS INC
- Keywords
- preeclampsia; amniotic fluid; SELDI; proapolipoprotein A-I; SBBI42
- Citation
- REPRODUCTIVE SCIENCES, v.15, no.5, pp.457 - 468
- Indexed
- SCIE
SCOPUS
- Journal Title
- REPRODUCTIVE SCIENCES
- Volume
- 15
- Number
- 5
- Start Page
- 457
- End Page
- 468
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/178646
- DOI
- 10.1177/1933719108316909
- ISSN
- 1933-7191
- Abstract
- Objective: To identify proteomic biomarkers in amniotic fluid (AF) that can distinguish preeclampsia (PE) from chronic hypertension (CHTN) and normotensive controls (CTR). Methods: AF from women with PE, CHTN, and CTR were subjected to proteomic analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Results: Proteomic profiling of AF identified 2 biomarkers: peak X (17399.11 Da), which distinguished PE from CTR, and peak Y (28023.34 Da), which distinguished PE and CHTN from CTR. High performance liquid chromatography fractions containing the biomarkers were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in-gel tryptic digestion. The biomarkers were matched to proapolipoprotein A-I (peak Y) and a functionally obscure peptide, SBBI42 (peak X). Western blot analysis confirmed that AF from PE and CHTN had higher proapolipoprotein A-I levels than CTR. Conclusion: Proteomic analysis of AF can distinguish PE from CHTN and CTR. The discriminatory proteins were identified as proapolipoprotein A-I and SBB142.
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