Neurochemical characterization of the TRPV1-Positive nociceptive, primary Afferents innervating skeletal muscles in the ratsopen access
- Authors
- Shin, Dong Su; Kim, Eun Hyun; Song, Kwan Young; Hong, Hyun Jong; Kong, Min Ho; Hwang, Se Jin
- Issue Date
- Feb-2008
- Publisher
- KOREAN NEUROSURGICAL SOC
- Keywords
- skeletal muscle; pain; primary afferents; TRPV1; CGRP; dorsal root ganglion.
- Citation
- JOURNAL OF KOREAN NEUROSURGICAL SOCIETY, v.43, no.2, pp.97 - 104
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF KOREAN NEUROSURGICAL SOCIETY
- Volume
- 43
- Number
- 2
- Start Page
- 97
- End Page
- 104
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179004
- DOI
- 10.3340/jkns.2008.43.2.97
- ISSN
- 2005-3711
- Abstract
- Objective : Transient receptor potential vanilloid subfamily type 1 (TRPV1), a most specific marker of the nociceptive primary afferent, is expressed in peptidergic and non-pepetidergic primary afferents innervating skin and viscera. However, its expression in sensory fibers to skeletal muscle is not well known. In this study, we studied the neurochemical characteristics of TRPV1-positive primary afferents to skeletal muscles. Methods : Sprague-Dawley rats were injected with total 20 mu l of 1% fast blue (FB) into the gastrocnemius and erector spinae muscle and animals were perfused 4 days after injection. FB-positive cells were traced in the L4-L5 (for gastrocnemius muscle) and L2-L4 (for erector spinae muscle) dorsal root ganglia. The neuro-chemical characteristics of the muscle afferents were studied with multiple immunofluorescence with TRPV1, calcitonin gene-related peptide ( CGRP) and P2X(3). To identify spinal neurons responding to noxious stimulus to the skeletal muscle, 10% acetic acids were injected into the gastrocnemius and erector spinae muscles and expression of phospho extracellular signal-regulated kinase (pERK) in spinal cords were identified with immunohistochemical method. Results: TRPV1 was expressed in about 49% of muscle afferents traced from gastrocnemius and 40% of erector spinae. Sixty-five to 60% of TRPV1-positive muscles afferents also expressed CGRP. In contrast, expression of P2X(3) immnoreaction in TRPV1-positive muscle afferents were about 20%. TRPV1-positive primary afferents were contacted with spinal neurons expressing pERK after injection of acetic acid into the muscles. Conclusion : It is consequently suggested that nociception from skeletal muscles are mediated by TRPV1-positive primary afferents and majority of them are also peptidergic.
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