Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate
- Authors
- Kim, Hyun Jeong; Bae, In Young; Ahn, Chang-Won; Lee, Suyong; Lee, Hyeon Gyu
- Issue Date
- Nov-2007
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- black soybean peptide; adipogenesis inhibitor; 3T3-L1 cell
- Citation
- PEPTIDES, v.28, no.11, pp.2098 - 2103
- Indexed
- SCIE
SCOPUS
- Journal Title
- PEPTIDES
- Volume
- 28
- Number
- 11
- Start Page
- 2098
- End Page
- 2103
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179369
- DOI
- 10.1016/j.peptides.2007.08.030
- ISSN
- 0196-9781
- Abstract
- Adipogenesis inhibitory peptide was isolated and identified from black soybean (Rhynchosia volubilis Lour.) hydrolysate. An adipogenesis inhibitor was purified using consecutive methods including: ultrafiltration (MWCO; 3 and 10 kDa), gel filtration chromatography (Superdex Peptide 10/300 GL column), and reverse-phase high-performance liquid chromatography (mu Bondapak(TM) C-18 column). Also, the adipogenesis inhibition effect of the purified peptide was measured by observation of droplet of 3T3-L1 adipocyte by Oil Red O staining in the highest active fraction in each step. The peptide was shown to inhibit the differentiation of the 3T3-L1 pre-adipocyte, which was confirmed by morphological study. The adipogenesis inhibitory peptide was purified 71.43-fold from black soybean hydrolysate throughout a five-step purification procedure. The adipogenesis inhibitor was identified to be a tripeptide, Ile-Gln-Asn, having an IC50 value of 0.014 mg protein/ml. Furthermore, the synthetic tripeptide (Ile-Gln-Asn) exhibited the similar adipogenesis effects to the purified peptide. Thus, these results showed the potential anti-obesity effect of the purified peptide through control of adiposity.
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