Phospholipase D1 activation through Src and Ras is involved in basic fibroblast growth factor-induced neurite outgrowth of H19-7 cells
- Authors
- Oh, Doo Yi; Park, Shin Young; Cho, Ju Hwan; Lee, Ki Sung; Min, Do Sik; Han, Joong Soo
- Issue Date
- May-2007
- Publisher
- WILEY-LISS
- Keywords
- phospholipase D1 (PLD1); neurite outgrowth; basic fibroblast growth factor (bFGF); Src; Ras; phospholipase; C-gamma (PLC-gamma)
- Citation
- JOURNAL OF CELLULAR BIOCHEMISTRY, v.101, no.1, pp.221 - 234
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CELLULAR BIOCHEMISTRY
- Volume
- 101
- Number
- 1
- Start Page
- 221
- End Page
- 234
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180176
- DOI
- 10.1002/jcb.21166
- ISSN
- 0730-2312
- Abstract
- Phospholipase D (PLD) is implicated in a variety of physiological processes that reveal it to be a member of the signal transducing phospholipases. We found that PLD1 is activated when basic fibroblast growth factor (bFGF) stimulates neurite outgrowth of an immortalized hippocampal cell line (H19-7). Overexpression of PLD1 in H19-7 cells dramatically elongated bFGF-induced neurite outgrowth and increased PLD activity. Transfection of DN-rPLD1 blocked bFGF-induced PLD activation and completely inhibited neurite outgrowth induced by bFGF, suggesting that PLD1 activation is important in bFGF-induced neurite outgrowth of H19-7 cells. PLD activation and neurite outgrowth induced by bFGF was dependent on phospholipase C gamma (PLC-gamma) and Ca2+, but not protein kinase C (PKC). Furthermore, inhibition of Src and Ras partially blocked bFGF-induced PLD activation and neurite outgrowth, respectively. Coinhibition of Src and Ras completely blocked bFGF-induced PLD activation, suggesting that Src and Ras independently regulate PLD1 activation. Interestingly, bFGF-induced PLD activation and neurite outgrowth did not require ERK1/2 activated by Ras. Taken together, this study demonstrates that bFGF activates PLD1 through PLC-gamma activation, which leads to neurite outgrowth in H19-7 cells. Furthermore, our results show that PLD1 activation by bFGF is regulated by Src and Ras independently.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의과대학 > 서울 생화학·분자생물학교실 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.