Direct determination of nucleosides in the urine of patients with breast cancer using column-switching liquid chromatography-tandem mass spectrometryopen access
- Authors
- Cho, Sung-Hee; Jung, Byung Hwa; Lee, Sang Hee; Lee, Won-Yong; Kong, Gu; Chung, Bong Chul
- Issue Date
- Nov-2006
- Publisher
- WILEY
- Keywords
- nucleosides; column-switching liquid chromatography; mass spectrometry; urine; breast cancer
- Citation
- BIOMEDICAL CHROMATOGRAPHY, v.20, no.11, pp.1229 - 1236
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMEDICAL CHROMATOGRAPHY
- Volume
- 20
- Number
- 11
- Start Page
- 1229
- End Page
- 1236
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180786
- DOI
- 10.1002/bmc.689
- ISSN
- 0269-3879
- Abstract
- We developed an analytical method for a simple, sensitive and simultaneous determination of oxidized nucleosides in urine using column-switching liquid chromatography-electrospray/tandem mass spectrometry (LC-ESI/MS/MS). We connected two columns through a six-way switching valve and effectively separated nucleosides in the urine from the interference by column-switching liquid chromatography. We monitored separated nucleosides using positive ionization tandem mass spectrometry in selective reaction monitoring (SRM) mode. The calibration ranges of nucleosides were 0.2-100 nmol/mL. The linearity of the method was 0.994-0.999, and the limits-of-detection (LOD) at a signal-to-noise (S/N) ratio of 3 were 0.1-0.2 nmol/mL. The coefficients of variation were in the range 2.28-11.74% for within-day variation and 4.36-11.15% for day-to-day variation, respectively. To explore the relationship between breast cancer and the nucleosides level in human urine, we measured the concentrations of nucleosides in female patients with breast cancer (n = 30) and in normal female subjects (n = 30). The concentration of nucleosides was significantly increased in patients with breast cancer when compared with the normal controls (1-methyladenosine; p < 0.005, N-2,N-2-dimethylguanosine; p < 0.01, 5-hydroxymethyl-2'-deoxyuridine; p < 0.001, 8-hydroxy-2-deoxyguanosine; p < 0.001). Therefore, the elevated levels of nucleosides could be used as an important biomarker for breastcancer research.
- Files in This Item
-
- Appears in
Collections - 서울 의과대학 > 서울 병리학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180786)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.