N-acetyl histidine-conjugated glycol chitosan self-assembled nanoparticles for intracytoplasmic delivery of drugs: Endocytosis, exocytosis and drug release
- Authors
- Park, Ji Sun; Han, Tae Hee; Lee, Kuen Yong; Han, Sung Soo; Hwang, Jung Jin; Moon, Dae Hyuk; Kim, Sang Yoon; Cho, Yong Woo
- Issue Date
- Sep-2006
- Publisher
- ELSEVIER
- Keywords
- nanoparticle; histidine; chitosan; endocytosis; exocytosis; endosome; paclitaxel; cell cycle arrest
- Citation
- JOURNAL OF CONTROLLED RELEASE, v.115, no.1, pp.37 - 45
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CONTROLLED RELEASE
- Volume
- 115
- Number
- 1
- Start Page
- 37
- End Page
- 45
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181076
- DOI
- 10.1016/j.jconrel.2006.07.011
- ISSN
- 0168-3659
- Abstract
- Nano-sized vesicular systems (nanoparticles), ranging from 10 nm to 1000 nm in size, have potential applications as drug delivery systems. Successful clinical applications require the efficient intracellular delivery of drug-loaded nanoparticles. Here we describe N-acetyl histidine-conjugated glycol chitosan (NAcHis-GC) self-assembled nanoparticles as a promising system for intracytoplasmic delivery of drugs. Because N-acetyl histidine (NAcHis) is hydrophobic at neutral pH, the conjugates formed self-assembled nanoparticles with mean diameters of 150-250 nm. In slightly acidic environments, such as those in endosomes, the nanoparticles were disassembled due to breakdown of the hydrophilic/hydrophobic balance by the protonation of the imidazole group of NAcHis. Cellular internalization and drug release of the pH-sensitive self-assembled nanoparticles were investigated by flow cytometry and confocal microscopy. NAcHis-GC nanoparticles internalized by adsorptive endocytosis were exocytosed or localized in endosomes. The amount of exocytosed nanoparticles was dependent on the pre-incubation time prior to removal of free nanoparticles from the culture media. Flow cytometry and confocal microscopy showed that NAcHis-GC nanoparticles released drugs into the cytosol and cell cycle analysis demonstrated that paclitaxel-incorporated NAcHis-GC nanoparticles were effective in inducing arrest of cell growth.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 공과대학 > 서울 생명공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181076)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.