NF-kappa B activation pathway is essential for the chemokine expression in intestinal epithelial cells stimulated with Clostridium difficile toxin Aopen access
- Authors
- Kim, Jung Mogg; Lee, Jin-Young; Yoon, Young Mee; Oh, Yu Kyoung; Youn, Jeungyeun; Kim, Young-Jeon
- Issue Date
- Jun-2006
- Publisher
- WILEY
- Citation
- SCANDINAVIAN JOURNAL OF IMMUNOLOGY, v.63, no.6, pp.453 - 460
- Indexed
- SCIE
SCOPUS
- Journal Title
- SCANDINAVIAN JOURNAL OF IMMUNOLOGY
- Volume
- 63
- Number
- 6
- Start Page
- 453
- End Page
- 460
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181354
- DOI
- 10.1111/j.1365-3083.2006.001756.x
- ISSN
- 0300-9475
- Abstract
- Intestinal epithelial cells are known to upregulate the expression of several chemokines in response to stimulation with bacterial toxin. However, the cellular mechanisms of Clostridium difficile toxin A-induced mucosal inflammation have not yet been fully elucidated. In this study, we investigated whether nuclear factor-kappa B (NF-kappa B) could regulate chemokine expression in intestinal epithelial cells. Toxin A increased the levels of NF-kappa B complexes containing p65/p50 heterodimers and p65/p65 homodimers. Concurrently, toxin A decreased the levels of I kappa B alpha. Toxin A stimulation also increased the signals of phosphorylated I kappa B kinase (IKK)alpha/beta and NF-kappa B-inducing kinase (NIK). In the toxin A-stimulated HT-29 cells, the suppression of IKK or NIK inhibited the upregulation of downstream target genes of NF-kappa B such as IL-8 and monocytechemotactic protein (MCP)-1 and similarly, inhibition of NF-kappa B also downregulated the expression of IL-8, growth-related oncogene-alpha, and MCP-1. These results suggest that NF-kappa B signalling events may be involved in the inflammatory responses to toxin A produced by toxigenic C. difficile.
- Files in This Item
-
- Appears in
Collections - 서울 의과대학 > 서울 해부·세포생물학교실 > 1. Journal Articles
- 서울 의과대학 > 서울 미생물학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181354)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.