Optimal temperature control in fractional precipitation for paclitaxel pre-purification
- Authors
- Jeon, Sun Im; Mun, Sung yong; Kim, Jin-Hyun
- Issue Date
- Feb-2006
- Publisher
- ELSEVIER SCI LTD
- Keywords
- paclitaxel; fractional precipitation; temperature; pre-purification
- Citation
- PROCESS BIOCHEMISTRY, v.41, no.2, pp.276 - 280
- Indexed
- SCIE
SCOPUS
- Journal Title
- PROCESS BIOCHEMISTRY
- Volume
- 41
- Number
- 2
- Start Page
- 276
- End Page
- 280
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181781
- DOI
- 10.1016/j.procbio.2005.07.016
- ISSN
- 1359-5113
- Abstract
- Fractional precipitation is a simple, efficient method for pre-purifying paclitaxel from plant cell cultures. The storage temperature of fractional precipitation was optimized in terms of the yield and purity of paclitaxel with a fixed methanol concentration in water (61.5%, v/v), paclitaxel content in the crude extract (0.5%, w/v), and storage time (3 days). The greatest yield (similar to 84%) was obtained with storage at a constant temperature (0 degrees C) for 3 days. Conversely, the highest purity (similar to 79%) was obtained with stepwise reduction in temperature over 3 days. For a constant storage temperature, the maximum purity (similar to 67%) was obtained at 0 degrees C. There was little difference in the yield of paclitaxel between -20 and 12 degrees C. At a constant storage temperature (0 degrees C), both the precipitate yield and purity increased rapidly up to 18 h, and then increased more slowly. This pre-purification process serves to minimize solvent usage, and the size and complexity of the high-performance liquid chromatography (HPLC) operation required for paclitaxel purification.
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