Polydiacelylene (PDA)-based colorimetric detection of biotin-streptavidin interactions
- Authors
- Jung, Yun Kyung; Park, Hyun Gyu; Kim, Jong Man
- Issue Date
- Feb-2006
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- polydiacetylene; biotin; streptavidin; colorimetric biosensors
- Citation
- BIOSENSORS & BIOELECTRONICS, v.21, no.8, pp.1536 - 1544
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 21
- Number
- 8
- Start Page
- 1536
- End Page
- 1544
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181808
- DOI
- 10.1016/j.bios.2005.07.010
- ISSN
- 0956-5663
- Abstract
- The natural binding of streptavidin (STA) for a small molecule, biotin, has made it a useful tool in specific targeting application, due to their most specific noncovalent biological interactions in nature. Here, we describe a polydiacetylene (PDA)-based colorimetric biosensor to detect the STA-biotin interactions. We synthesized two kinds of biotin-labeled 10,12-pentacosadiynoic acid (PCDA) monomers having 2(ethylene oxide) or 3(ethylene oxide) spacer and prepared biotin-modified PDA liposomes using them. Upon the addition of STA, the modified PDA liposomes showed a color change from blue to red in about I h followed by their aggregation and precipitation. The aggregation was found to be due to the cross-linking between the PDA liposomes caused by the STA having four biotin binding sites, which was confirmed by their TEM analysis. Since the STA-biotin interactions are widely adopted for various biological detection systems; the biotin-modified PDA sensor developed in this study has enormous potentials for the development of various colorimetric biosensors.
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