Circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse modelopen access
- Authors
- Seo, Young Joon; Shim, Dae Bo; Sharif, Arazu; Samson, Zeke; Takechi, Ryu; Brown, Daniel
- Issue Date
- Oct-2022
- Publisher
- WILEY
- Keywords
- db; db mice; diabetes mellitus; hearing loss; light sheet microscopy; spiral modiolar artery
- Citation
- LARYNGOSCOPE INVESTIGATIVE OTOLARYNGOLOGY, v.7, no.5, pp.1568 - 1574
- Indexed
- SCIE
SCOPUS
- Journal Title
- LARYNGOSCOPE INVESTIGATIVE OTOLARYNGOLOGY
- Volume
- 7
- Number
- 5
- Start Page
- 1568
- End Page
- 1574
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/186121
- DOI
- 10.1002/lio2.917
- ISSN
- 2378-8038
- Abstract
- Objective This study aimed to identify significant differences in cochlea microvessel size between a diabetic mouse model (db/db) and normal mice using three-dimensional (3D) quantitative analysis. Methods Six control heterozygote db/+ as well as 18 male B6/BKS(D)-Lepr(db)/J (db/db) mice aged 14 (n = 9) and 28 (n = 9) weeks were examined. After clearing the cochlea, we reconstructed the 3D volumes of the spiral modiolar artery (SMA) in the cochlea using light-sheet microscopy and analyzed vessel wall thickness, cross-sectional area, short and long diameter, and vessel height. Results The average SMA-wall thickness in the db/db-mouse group (3.418 +/- 0.328 mu m) was greater than that in the control group (2.388 +/- 0.411 mu m). The average cross-sectional outer area, short diameter, and long diameter of the SMA in db/db mice were significantly larger than those in control mice (all p < 0.001). The cross-sectional areas increased with age (control: 221.782 +/- 121.230 mu m, 14 weeks; 294.378 +/- 151.008 mu m, and 28 weeks; 312.925 +/- 147.943 mu m). Conclusion The db/db mice had thicker and larger proximal-SMA vessel walls and diameters than control mice, respectively, thus potentially inducing increased blood viscosity or vascular insufficiency and aggravating hearing loss in type 2 diabetes mellitus. Level of Evidence IIb
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