Development of Lactobacillus kimchicus DCY51T-mediated gold nanoparticles for delivery of ginsenoside compound K: in vitro photothermal effects and apoptosis detection in cancer cellsopen access
- Authors
- Kim, Yeon-Ju; Perumalsamy, Haribalan; Markus, Josua; Balusamy, Renukadevi; Wang, Chao; Kang, Seong Ho; Lee, Seungah; Park, Sang Yong; Kim, Sung; Castro-Aceituno, Veronica; Kim, Seung Hyun; Yang, Deok Chun
- Issue Date
- Dec-2019
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Ginsenoside CK; gold nanoparticles; photothermal therapy; photoluminescence; drug delivery; green synthesis; anticancer activity
- Citation
- Artificial Cells, Nanomedicine, and Biotechnology, v.47, no.1, pp.30 - 44
- Indexed
- SCIE
SCOPUS
- Journal Title
- Artificial Cells, Nanomedicine, and Biotechnology
- Volume
- 47
- Number
- 1
- Start Page
- 30
- End Page
- 44
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/189829
- DOI
- 10.1080/21691401.2018.1541900
- ISSN
- 2169-1401
- Abstract
- We report a non-covalent loading of ginsenoside compound K (CK) onto our previously reported gold nanoparticles (DCY51(T)-AuCKNps) through one-pot biosynthesis using a probiotic Lactobacillus kimchicus DCY51(T) isolated from Korean kimchi. The ginsenoside-loaded gold nanoparticles were characterized by various analytical and spectroscopic techniques such as field emission transmission electron microscopy (FE-TEM), energy-dispersive X-ray (EDX) spectroscopy, elemental mapping, X-ray powder diffraction (XRD), selected area electron diffraction (SAED), Fourier-transform infrared (FTIR) spectroscopy and dynamic light scattering (DLS). Furthermore, drug loading was also determined by liquid chromatography-mass spectrometry (LC-MS). In addition, DCY51(T)-AuNps and DCY51(T)-AuCKNps were resistant to aggregation caused by pH variation or a high ionic strength environment. Cell-based study confirmed that DCY51(T)-AuCKNps exhibited slightly higher cytotoxicity compared to ginsenoside CK treatment in A549 cells (human lung adenocarcinoma cell line) and HT29 (human colorectal adenocarcinoma cell line). Upon laser treatment, DCY51(T)-AuCKNps showed enhanced cell apoptosis in A549, HT29 and AGS cells (human stomach gastric adenocarcinoma cell line) compared with only DCY51(T)-AuCKNps treated cells. In conclusion, this preliminary study identified that DCY51(T)-AuCKNps act as a potent photothermal therapy agents with synergistic chemotherapeutic effects for the treatment of cancer.
- Files in This Item
-
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/189829)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.