Intracellular synthesis of gold nanoparticles by Gluconacetobacter liquefaciens for delivery of peptide CopA3 and ginsenoside and anti-inflammatory effect on lipopolysaccharide-activated macrophagesopen access
- Authors
- Liu, Ying; PERUMALSAMY HARIBALAN; Kang, Chang Ho; Kim, Seung Hyun; Hwang, Jae-Sam; Koh, Sung-Cheol; Yi, Tae-Hoo; Kim, Yeon-Ju
- Issue Date
- Apr-2020
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Peptide CopA3; Compound K; gold nanoparticles; anti-inflammatory; NF-kappa B
- Citation
- ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, v.48, no.1, pp.777 - 788
- Indexed
- SCIE
SCOPUS
- Journal Title
- ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
- Volume
- 48
- Number
- 1
- Start Page
- 777
- End Page
- 788
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/191415
- DOI
- 10.1080/21691401.2020.1748639
- ISSN
- 2169-1401
- Abstract
- Probiotic Gluconacetobacter strains are intestinal microbes with beneficial effects on human health. Recently, researchers have used these strains to biosynthesize metal and non-metal nanoparticles for treating various chronic diseases. Despite their importance in nanotechnology, gold nanoparticles (AuNPs) biosynthesized by Gluconacetobacter species have not been clearly identified for treating inflammation and inflammation-associated diseases. While ginsenoside CK has strong pharmaceutical activity, it also has strong cytotoxicity and hydrophobicity which is hurdle to make formulation. Peptide-nanoparticle hybrids are gaining increasing attention for their potential biomedical applications, including human inflammatory diseases. Herein, we developed peptide CopA3 surface conjugated and ginsenoside compound K (CK) loaded gold nanoparticles (GNP-CK-CopA3), which intracellularly synthesised by the probiotic Gluconacetobacter liquefaciens kh-1, to target lipopolysaccharide (LPS)-activated RAW264.7 macrophages. The synthetic GNP-CK-CopA3 was characterised by various instrumental techniques. The results of our cellular uptake and MTT assays exhibited obvious drug intracellular delivery without significant cytotoxicity. In addition, pre-treatment with GNP-CK-CopA3 significantly ameliorated LPS-induced nitric oxide (NO) and reactive oxygen species (ROS) production and suppressed the mRNA and protein expression of pro-inflammatory cytokines in macrophages. Furthermore, GNP-CK-CopA3 efficiently inhibited the activation of the nuclear factor-kappa B (NF-kappa B) and mitogen-activating protein kinase (MAPK) signalling pathways. Taken together, our findings highlight the potential of using peptide-nanoparticle hybrids in the development of anti-inflammatory approaches and providing the experimental foundation for further application.
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