Photosensitizing deep-seated cancer cells with photoprotein-conjugated upconversion nanoparticlesopen access
- Authors
- Park, Sung Hyun; Han, Soohyun; Park, Sangwoo; Kim, Hyung Shik; Kim, Kyung-Min; Kim, Suyeon; Lee, Dong Yun; Lee, Joonseok; Kim, Young-Pil
- Issue Date
- Aug-2023
- Publisher
- BioMed Central Ltd
- Keywords
- Photodynamic therapy; Protein photosensitizers; Upconversion nanoparticles; Reactive oxygen species; Near-infrared light
- Citation
- Journal of Nanobiotechnology, v.21, no.1, pp 1 - 17
- Pages
- 17
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Nanobiotechnology
- Volume
- 21
- Number
- 1
- Start Page
- 1
- End Page
- 17
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192956
- DOI
- 10.1186/s12951-023-02057-0
- ISSN
- 1477-3155
1477-3155
- Abstract
- To resolve the problem of target specificity and light transmission to deep-seated tissues in photodynamic therapy (PDT), we report a cancer cell-targeted photosensitizer using photoprotein-conjugated upconversion nanoparticles (UCNPs) with high target specificity and efficient light transmission to deep tissues. Core-shell UCNPs with low internal energy back transfer were conjugated with recombinant proteins that consists of a photosensitizer (KillerRed; KR) and a cancer cell-targeted lead peptide (LP). Under near infrared (NIR)-irradiating condition, the UCNP-KR-LP generated superoxide anion radicals as reactive oxygen species via NIR-to-green light conversion and exhibited excellent specificity to target cancer cells through receptor-mediated cell adhesion. Consequently, this photosensitizing process facilitated rapid cell death in cancer cell lines (MCF-7, MDA-MB-231, and U-87MG) overexpressing integrin beta 1 (ITGB1) receptors but not in a cell line (SK-BR-3) with reduced ITGB1 expression and a non-invasive normal breast cell line (MCF-10A). In contrast to green light irradiation, NIR light irradiation exhibited significant PDT efficacy in cancer cells located beneath porcine skin tissues up to a depth of 10 mm, as well as in vivo tumor xenograft mouse models. This finding suggests that the designed nanocomposite is useful for sensing and targeting various deep-seated tumors.
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